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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
14/04/2009 |
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Data da última atualização: |
30/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COELHO, M. R. R.; MARRIEL, I. E.; JENKINS, S. N.; LANYON, C. V.; SELDIN, L.; O'DONNELL, A. G. |
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Afiliação: |
Márcia R. R. Coelho, UFRJ; IVANILDO EVODIO MARRIEL, CNPMS; Sasha N. Jenkins, Newcastle University; Clare V. Lanyon, Newcastle University; Lucy Seldin, UFRJ; Anthony G. O'Donnell, Newcastle University. |
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Título: |
Molecular detection and quantification of nifH gene sequences in the rhizosphere of sorghum (Sorghum bicolor) sown with two levels of nitrogen fertilizer. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Applied Soil Ecology, Amsterdam, v. 42, n. 1, p. 48-53, 2009. |
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DOI: |
10.1016/j.apsoil.2009.01.010 |
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Idioma: |
Inglês |
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Conteúdo: |
Denaturing gradient gel electrophoresis (DGGE) and SYBR Green I quantitative real-time PCR (qPCR) approaches were used to assess respectively the molecular diversity and the quantity of the nifH gene sequences in the rhizospheres of two cultivars of sorghum sown in Cerrado soil with contrasting levels of nitrogen fertilizer. DGGE fingerprinting showed that for both cultivars the presumptive nitrogen-fixing populations in the rhizospheres were more diverse than in bulk soil. Sequencing of nifH gene fragments obtained from DGGE bands revealed that members of the order Rhizobiales were prevalent among the dominant diazotrophs. Discriminant analysis of DGGE profiles resulted into three groups formed by (i) cultivar BRS 308 sown with high level of nitrogen, (ii) cultivar BRS 308 sown with low level of nitrogen and cultivar BRS 310 sown either with low or high levels of nitrogen and (iii) bulk soil, showing that the nitrogen fertilization influenced the nifH gene sequence diversity only in the rhizosphere of cultivar BRS 308. When the quantity of the nifH gene sequences was determined by q-PCR, 2.4 x105 to 1.3 x 107 copies/g of soil were detected. The highest number of nifH gene copies was observed in the rhizosphere of cultivar BRS 308 treated with low amount of fertilizer, and a reduction in nifH density was observed in the rhizospheres of both sorghum cultivars when high levels of nitrogen were applied. Thus, both the amount of nitrogen fertilizer and the cultivar are important factors influencing the structure and amount of diazotrophs in sorghum. MenosDenaturing gradient gel electrophoresis (DGGE) and SYBR Green I quantitative real-time PCR (qPCR) approaches were used to assess respectively the molecular diversity and the quantity of the nifH gene sequences in the rhizospheres of two cultivars of sorghum sown in Cerrado soil with contrasting levels of nitrogen fertilizer. DGGE fingerprinting showed that for both cultivars the presumptive nitrogen-fixing populations in the rhizospheres were more diverse than in bulk soil. Sequencing of nifH gene fragments obtained from DGGE bands revealed that members of the order Rhizobiales were prevalent among the dominant diazotrophs. Discriminant analysis of DGGE profiles resulted into three groups formed by (i) cultivar BRS 308 sown with high level of nitrogen, (ii) cultivar BRS 308 sown with low level of nitrogen and cultivar BRS 310 sown either with low or high levels of nitrogen and (iii) bulk soil, showing that the nitrogen fertilization influenced the nifH gene sequence diversity only in the rhizosphere of cultivar BRS 308. When the quantity of the nifH gene sequences was determined by q-PCR, 2.4 x105 to 1.3 x 107 copies/g of soil were detected. The highest number of nifH gene copies was observed in the rhizosphere of cultivar BRS 308 treated with low amount of fertilizer, and a reduction in nifH density was observed in the rhizospheres of both sorghum cultivars when high levels of nitrogen were applied. Thus, both the amount of nitrogen fertilizer and the cultivar are important... Mostrar Tudo |
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Thesagro: |
Sorgo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53597/1/Molecular-detection.pdf
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Marc: |
LEADER 02260naa a2200205 a 4500
001 1491948
005 2018-05-30
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1016/j.apsoil.2009.01.010$2DOI
100 1 $aCOELHO, M. R. R.
245 $aMolecular detection and quantification of nifH gene sequences in the rhizosphere of sorghum (Sorghum bicolor) sown with two levels of nitrogen fertilizer.$h[electronic resource]
260 $c2009
520 $aDenaturing gradient gel electrophoresis (DGGE) and SYBR Green I quantitative real-time PCR (qPCR) approaches were used to assess respectively the molecular diversity and the quantity of the nifH gene sequences in the rhizospheres of two cultivars of sorghum sown in Cerrado soil with contrasting levels of nitrogen fertilizer. DGGE fingerprinting showed that for both cultivars the presumptive nitrogen-fixing populations in the rhizospheres were more diverse than in bulk soil. Sequencing of nifH gene fragments obtained from DGGE bands revealed that members of the order Rhizobiales were prevalent among the dominant diazotrophs. Discriminant analysis of DGGE profiles resulted into three groups formed by (i) cultivar BRS 308 sown with high level of nitrogen, (ii) cultivar BRS 308 sown with low level of nitrogen and cultivar BRS 310 sown either with low or high levels of nitrogen and (iii) bulk soil, showing that the nitrogen fertilization influenced the nifH gene sequence diversity only in the rhizosphere of cultivar BRS 308. When the quantity of the nifH gene sequences was determined by q-PCR, 2.4 x105 to 1.3 x 107 copies/g of soil were detected. The highest number of nifH gene copies was observed in the rhizosphere of cultivar BRS 308 treated with low amount of fertilizer, and a reduction in nifH density was observed in the rhizospheres of both sorghum cultivars when high levels of nitrogen were applied. Thus, both the amount of nitrogen fertilizer and the cultivar are important factors influencing the structure and amount of diazotrophs in sorghum.
650 $aSorgo
700 1 $aMARRIEL, I. E.
700 1 $aJENKINS, S. N.
700 1 $aLANYON, C. V.
700 1 $aSELDIN, L.
700 1 $aO'DONNELL, A. G.
773 $tApplied Soil Ecology, Amsterdam$gv. 42, n. 1, p. 48-53, 2009.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Origem |
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Classificação |
Cutter |
Registro |
Volume |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
06/01/2015 |
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Data da última atualização: |
06/01/2015 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
TAVARES, L. C. V.; BASSOI, M. C.; FOLONI, J. S.; PRANDO, A. M.; CONTE, O.; SILVA FILHO, P. M.; OLIVEIRA, A. B.; LIMA, D.; BORGES, R. S.; BECKERT, O. P.; DALBOSCO, M.; DENGLER, R. U. |
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Afiliação: |
LUÍS CÉSAR VIEIRA TAVARES, CNPSO; MANOEL CARLOS BASSOI, CNPSO; JOSÉ SALVADOR SIMONETI FOLONI, CNPSO; ANDRÉ MATEUS PRANDO, CNPSO; OSMAR CONTE, CNPSO; PEDRO MOREIRA DA SILVA FILHO, CNPSO; ARNOLD BARBOSA DE OLIVEIRA, CNPSO; DIVANIA DE LIMA, CNPSO; ROGÉRIO DE SÁ BORGES, SPM E Londrina; OSMAR PAULO BECKERT, SPM E Ponta Grossa; Fundação Meridional de Apoio à Pesquisa Agropecuária; Fundação Meridional de Apoio à Pesquisa Agropecuária. |
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Título: |
Apresentação das cultivares de trigo da Embrapa, na Região Meridional, safra 2013. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 8.; SEMINÁRIO TÉCNICO DE TRIGO, 9., 2014, Canela. Desafios para o trigo brasileiro: construindo uma nova identidade: anais. Passo Fundo: Embrapa Trigo, 2014. |
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Páginas: |
3 p. |
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Idioma: |
Português |
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Thesagro: |
Trigo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114809/1/Apresentacao-das-cultivares-de-trigo-da-Embrapa-na-Regiao-Meridional-safra-2013.pdf
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Marc: |
LEADER 00899nam a2200253 a 4500
001 2004320
005 2015-01-06
008 2014 bl uuuu u00u1 u #d
100 1 $aTAVARES, L. C. V.
245 $aApresentação das cultivares de trigo da Embrapa, na Região Meridional, safra 2013.$h[electronic resource]
260 $aIn: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 8.; SEMINÁRIO TÉCNICO DE TRIGO, 9., 2014, Canela. Desafios para o trigo brasileiro: construindo uma nova identidade: anais. Passo Fundo: Embrapa Trigo$c2014
300 $a3 p.
650 $aTrigo
700 1 $aBASSOI, M. C.
700 1 $aFOLONI, J. S.
700 1 $aPRANDO, A. M.
700 1 $aCONTE, O.
700 1 $aSILVA FILHO, P. M.
700 1 $aOLIVEIRA, A. B.
700 1 $aLIMA, D.
700 1 $aBORGES, R. S.
700 1 $aBECKERT, O. P.
700 1 $aDALBOSCO, M.
700 1 $aDENGLER, R. U.
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Registro original: |
Embrapa Soja (CNPSO) |
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